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DS Technology Platforms
Flow Chemistry Synthetic Biology & Biocatalysis Highly Potent Compound Peptide TPD/PROTAC Oligonucleotide Synthesis Crystallization & Particle Engineering Preparation & Separation
Synthetic Biology & Biocatalysis Platform
Apeloa has established a comprehensive synthetic biology platform to support the development of efficient and robust non-GMP and GMP-compliant bio-production processes. With over 20 years of experience in bio-production technologies, including fermentation and enzyme production, Apeloa CDMO is well-positioned to handle complex bio-production projects. The platform is supported by state-of-the-art laboratories and an experienced team of highly educated bio-scientists and engineers.
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Team
The synthetic biology and biocatalysis R&D team, located at Geshan site, comprises more than 70 scientists. The team includes dedicated experts in strain engineering, biotransformation, fermentation, and downstream processing (DSP). Led by experienced scientists, the team has a proven track record in developing industrial viable biocatalysts and delivering successful bio-production processes.
Services
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尊龙凯时药业
尊龙凯时药业
尊龙凯时药业
尊龙凯时药业
Apeloa's synthetic biology platform offers a comprehensive range of services, including: ●Microbial strain improvement and optimization ●Fermentation process development and optimization ●Separation and downstream processing (DSP) ●Biotransformation and enzyme engineering ●Synthetic biology techniques (e.g., gene editing, high-throughput screening)
Equipment
The platform is equipped with state-of-the-art facilities/equipment, including:
  • Gel imaging system
    Gel imaging system
  • High throughput shaker
    High throughput shaker
  • Parallel reactor
    Parallel reactor
  • PCR
    PCR
  • Microplate reader
    Microplate reader
  • GC/GCMS
    GC/GCMS
  • LCMS
    LCMS
  • Rapidfire HT MS
    Rapidfire HT MS
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Gel imaging system
尊龙凯时药业
High throughput shaker
尊龙凯时药业
Parallel reactor
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PCR
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Microplate reader
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GC/GCMS
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LCMS
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Rapidfire HT MS
Case 1
Case 2
Case 3
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Engineering of amino transferase
● In-house library of amino transferases were screened for the target reaction. ● The best hit gave the desired product enantiomer but with low yield (30% for 10 mM substrate & 100 mM alanine). ● Mutagenesis in the enzyme’s substrate pocket did not improve the activity. ● Random mutagenesis with ep-PCR in combination of RapidFire 460 HT mass spectrometry system resulted in a variant with 5 times higher activity
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Enzyme engineering for selective nitrile hydrolysis
● In-house nitrilases were screened against the target reaction. Two were found with moderate activity for the hydrolysis of terminal nitrile group. ● The structure of the enzymes were obtained via homologous modeling to help identify substrate pockets. ● The residues in the pocket were subjected to mutagenesis and screening (shown at right). Seventeen variants with 4-11 times improved activity was obtained. ● A variant showed. ● Process was developed to scale up the reaction in 40 kg scale, affording 3 kg product with 99% ee and 73% yield (substrate was recycled via racemization).
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Enzyme screening
● Screening of in-house lipases and esterases to find an active enzyme for the kinetic resolution of a racemic alcohol via esterification. ● The reaction was transferred to chemical process team for scale-up and integration with downstream derivatization.
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